In the BioVendor Human S100 a12 ELISA Kit, the standards, superior controls and samples are incubated in microtitrate wells pre-coated with polyclonal anti-human S100A12 antibody.
After 60 min evolution and a washing, biotin-labelled polyclonal anti-human S100A12 antibiotic is added and incubated with captured S100A12 for 60 min. After accession washing, the streptavidin-HRP conjugate is added. After 30 min evolution and the endure abrasion step, the actual conjugate is accustomed to acknowledge with the substrate band-aid (TMB). The acknowledgment is chock-full by accession of acerb solution, and absorbance of the consistent chicken artefact is measured. The absorbance is proportional to the absorption of S100A12. A accepted ambit is complete by acute absorbance ethics adjoin concentrations of standards, and concentrations of alien samples are bent application this accepted curve.